Aminotransferases in Peroxisomes from Spinach Leaves
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چکیده
منابع مشابه
Aminotransferases in peroxisomes from spinach leaves.
Organelles in spinach leaves were isolated in sucrose gradients by isopycnic centrifugation. The peroxisomal fraction was the only cellular site for two different and irreversible aminotransferases which utilized glyoxylate as the amino acceptor in the formation of glycine. These were a serine:glyoxylate aminotransferase with a specific activity of 1.54 pmoles X min-1 X mg-1 peroxisomal protein...
متن کاملOxidation of formate by peroxisomes and mitochondria from spinach leaves.
1. Spinach (Spinacia oleracea L.) leaf extracts catalyse the oxidation of formate to CO(2). 2. Two enzymic systems are responsible for this oxidation, the peroxidatic action of catalase (EC 1.11.1.6) and NAD-dependent formate dehydrogenase (EC 1.2.1.2). 3. Formate dehydrogenase is mainly, if not exclusively, located in the mitochondria. This enzyme has a pH optimum of 6-6.5 and a K(m) for forma...
متن کاملPeroxisomes from spinach leaves containing enzymes related to glycolate metabolism.
Microbodies, designated as peroxisomes because of their enzyme complement, have been isolated from spinach leaves. After grinding leaves in 0.5 M sucrose, the peroxisomes were removed with the broken chloroplast fraction by differential centrifugation. During sucrose density gradient centrifugation, the peroxisomes banded in about 1.9 M sucrose and were separated from mitochondria and chloropla...
متن کاملParticipation of mitochondrial metabolism in photorespiration. Reconstituted system of peroxisomes and mitochondria from spinach leaves
In this study the interplay of mitochondria and peroxisomes in photorespiration was simulated in a reconstituted system of isolated mitochondria and peroxisomes from spinach (Spinacia oleracea L.) leaves. The mitochondria oxidizing glycine produced serine, which was reduced in the peroxisomes to glycerate. The required reducing equivalents were provided by the mitochondria via the malate-oxaloa...
متن کاملPhosphatidic Acid and Glyceride Synthesis by Particles from Spinach Leaves.
For many years it has been recognized that particulate bodies within cells capable of carrying out energy transductions contain large quantities of lipids which include significant amounts of phosphatides, glycolipids, quinones, pigments and certain lipid soluble vitamins. A great deal of conjecture has been written concerning the possible role of lipids, especially those with amphipathic prope...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1972
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)44982-x